Dr. Nasim Mayeli | cell therapy | Best Researcher Award

Research Assistant at cell therapy , Shahid chamran university, Iran

Nasim Mayeli Fereydani earned her Ph.D. in Molecular Genetics from Shahid Chamran University in Ahvaz, Iran. Her doctoral research, conducted from 2016 to 2024, centered on correcting the Beta-globin CD36/37(-T) mutation in human hematopoietic stem cells using the CRISPR/Cas9 platform. Dr. Fereydani’s work was conducted under the guidance of Dr. Hamid Galehdari, a Professor in Molecular Genetics.

Professional Profiles:

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EDUCATION:

Nasim Mayeli Fereydani has a diverse academic background, starting with a B.Sc. in Microbiology from Shahid Chamran University in Ahvaz, Iran, completed from 2003 to 2007. She then pursued an M.Sc. in Microbiology at the same university, completing her thesis on the isolation of polyhydroxyalkanoates-producing bacteria from contaminated soil of Bandar Imam Petrochemical Industries under the supervision of Dr. Hossein Motamedi, a Professor in Microbiology, from 2009 to 2012. Nasim furthered her studies with a Ph.D. in Molecular Genetics at Shahid Chamran University, focusing her research on correcting the Beta-globin CD36/37(-T) mutation in human hematopoietic stem cells using the CRISPR/Cas9 platform. Her doctoral thesis was supervised by Dr. Hamid Galehdari, a Professor in Molecular Genetics, and she completed her Ph.D. from 2016 to 2024.

WORK EXPERIENCE:

Nasim Mayeli Fereydani has been actively involved in various educational, community, clinical, and research activities. As a Lecturer at Islamic Azad University Ahvaz Branch from 2021 to 2023, she taught courses on Principles of Genetics, Cancer Genetics, Genetic Engineering Laboratory, Basic Genetic Laboratory, and Cell Culture. In addition, she participated in professional community activities, focusing on disability awareness and prevention plan and familiarization with genetic counseling for students of Ahvaz, Iran-National Welfare Organization, from 2017 to 2018. In clinical settings, Nasim worked at Ghandil Medical Genetics Laboratory from 2013 to 2017. She served as the Supervisor and technical technician of the prenatal diagnostic of thalassemia department from 2015 to 2017, the technical technician of the molecular department from 2014 to 2017, and the technical technician of the cytogenetics department, involved in karyotyping, from 2013 to 2014. Furthermore, Nasim was a Research Assistant at Ahvaz Jundishapur University of Medical Sciences from 2011 to 2013. She collaborated on research projects in the faculty of medicine, department of immunology, student and research projects in the faculty of dentistry, and the research project of the thalassemia & hemoglobinopathy research center from 2019 to 2023.

SKILLS:

Nasim Mayeli Fereydani has a wide range of skills in both wet and dry lab techniques. In the wet lab, she is proficient in DNA and RNA extraction from various sources, including blood, cell lines, and bacteria. She has expertise in various PCR techniques, such as Conventional PCR, Real-time PCR (SYBR Green -TaqMan), ARMS-PCR, Multiplex-PCR, and PCR-RFLP. Nasim is also skilled in electrophoresis using agarose gel and Polyacrylamide (PAGE), with experience in silver nitrate staining. She is knowledgeable in cloning methods, including Conventional cloning and TA-cloning, as well as gene editing using CRISPR/Cas9. Nasim has experience in bacterial culture, mammalian cell culture (including cancer cell lines like A549, MCF7, HepG2, and HEK293T), and stem cell culture (isolating mesenchymal stem cells from various tissues and hematopoietic stem cells using immunomagnetic separation). She is also familiar with flow cytometry (sample preparation, instrument setting, and data analysis using FlowJo), fluorescent microscopy, cell transfection methods (Nucleofection and polyethylenimine), and laboratory animal handling (injection of cancer cells to create mouse models). In the dry lab, Nasim is skilled in primer and probe design for guide RNA and Donor DNA, statistical analysis using SPSS, and proposal writing.

PUBLICATIONS:

Mayeli N, Motamedi H, Heidarizadeh F. “Production of Polyhydroxybutyrate by Bacillus axaraqunsis BIPC01 using Petrochemical Wastewater as Carbon Source.” Braz arch biol technol. 2015:643-50

Motamedi H, Roayaee Ardakani M, Mayeli N. “Isolation and screening of native polyhydroxyalkanoate producing bacteria from oil contaminated soils of Abadan refinery.” Biological Journal of Microorganism. 2015;3(12):93-104.

Mayeli N, Galehdari H, Hoveizi E, Ajami M. “A comparison of the efficiency of the T7-Endonuclease I enzyme and polyacrylamide gel electrophoresis system in evaluating the cleavage efficiency of the CRISPR/Cas9 system.” Journal of Isfahan Medical School. 2024.

Mayeli N, Galehdari H, Hoveizi E, Ajami M, Alghasi A. “Ex vivo expansion of Hematopoietic stem cells in two/three-dimensional co-cultures with various sources of stromal cells.” Tissue and Cell journal, 2024 (accepted/in press).

“Genetic correction of CD 36/37 (-T) β-thalassemia mutation by CRISPR/Cas9 in human hematopoietic stem cells as a common mutation of southwest of Iran” (under preparation).