Nasim Mayeli Fereydani has been actively involved in various educational, community, clinical, and research activities. As a Lecturer at Islamic Azad University Ahvaz Branch from 2021 to 2023, she taught courses on Principles of Genetics, Cancer Genetics, Genetic Engineering Laboratory, Basic Genetic Laboratory, and Cell Culture. In addition, she participated in professional community activities, focusing on disability awareness and prevention plan and familiarization with genetic counseling for students of Ahvaz, Iran-National Welfare Organization, from 2017 to 2018. In clinical settings, Nasim worked at Ghandil Medical Genetics Laboratory from 2013 to 2017. She served as the Supervisor and technical technician of the prenatal diagnostic of thalassemia department from 2015 to 2017, the technical technician of the molecular department from 2014 to 2017, and the technical technician of the cytogenetics department, involved in karyotyping, from 2013 to 2014. Furthermore, Nasim was a Research Assistant at Ahvaz Jundishapur University of Medical Sciences from 2011 to 2013. She collaborated on research projects in the faculty of medicine, department of immunology, student and research projects in the faculty of dentistry, and the research project of the thalassemia & hemoglobinopathy research center from 2019 to 2023.

SKILLS:

Nasim Mayeli Fereydani has a wide range of skills in both wet and dry lab techniques. In the wet lab, she is proficient in DNA and RNA extraction from various sources, including blood, cell lines, and bacteria. She has expertise in various PCR techniques, such as Conventional PCR, Real-time PCR (SYBR Green -TaqMan), ARMS-PCR, Multiplex-PCR, and PCR-RFLP. Nasim is also skilled in electrophoresis using agarose gel and Polyacrylamide (PAGE), with experience in silver nitrate staining. She is knowledgeable in cloning methods, including Conventional cloning and TA-cloning, as well as gene editing using CRISPR/Cas9. Nasim has experience in bacterial culture, mammalian cell culture (including cancer cell lines like A549, MCF7, HepG2, and HEK293T), and stem cell culture (isolating mesenchymal stem cells from various tissues and hematopoietic stem cells using immunomagnetic separation). She is also familiar with flow cytometry (sample preparation, instrument setting, and data analysis using FlowJo), fluorescent microscopy, cell transfection methods (Nucleofection and polyethylenimine), and laboratory animal handling (injection of cancer cells to create mouse models).  In the dry lab, Nasim is skilled in primer and probe design for guide RNA and Donor DNA, statistical analysis using SPSS, and proposal writing.

PUBLICATIONS: